Laboratory skills prerequisites
To work at the lab without supervision you need to have previous practice in performing the majority of procedures listed below.
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Use single volume pipettor to work with 1-1000 ul volumes.
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Use serological pipettor to work with 1-100 ml volumes.
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Use small centrifuge which fits 1.5 ml tubes.
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Use big refrigerated centrifuge which fits 50 ml tubes.
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Properly label and store tubes with chemicals.
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Properly label and store plates and tubes with live bacteria.
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Prepare LB media, autoclave it, add antibiotic, and pour the plates.
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Prepare LB broth.
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Streak bacteria on plates. Use gas burner.
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Incubate plates in usual incubator.
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Incubate bacteria in liquid media in shaking incubator.
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Run PCR and qPCR.
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Pour gel for electrophoresis. Run electrophoresis.
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Calculate how much water has to be added to a solution to achieve intended dilution.
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Cleaning up after completion of an experiment.
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Disposal of biological and non-biological waste.
Classes
We run periodic Laboratory Skills Bootcamp, which covers all the basic laboratory skills. Please, check www.meetup.com/boslab to find when the next class is scheduled.